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KMID : 0350519930460041619
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Journal of Catholic Medical College
1993 Volume.46 No. 4 p.1619 ~ p.1630
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The Proliferation and Antitumor Effect of Tumor Infiltrating Lymphocytes and Tumor Draining Lymph Node Lymphocytes in Human Cervical Cancer
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Abstract
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To evaluate the immunological characteristics of tumer infiltration lymphocytes(TIL) and autologous peripheral blood lymphocytes(A-PBL) from 20 patients with primary cervical tumors and tumor draining lymph node lymphocytes(LNL) from 10 of them,
lymphocytes were cultred in vitro for proliferation, pheotyping and cytotoxicity.
Freshly obtained cervical tumor masses and draining lymph nodes were digented enzymatically. Lymphocytes were separated using ficoll-hypaque density gradient method, and were cultured in Waymouth´s MB 752/1 media with 5% human AB type serum only
or
with
1,000 U/ml recombinant interleukin-2(rIL-2).
@ES The results were as follows :
@EN 1. Lymphocytes from primary cervical tumors constituted from 20 % to 90 % of single cell tumor suspensions(average lymphocyte : tumor cell=0.95) and expanded from 2.6-folds to 130-folds in 12 of 20 cultures. For 10 of 20 patients, lymphocytes
derived from tumor draining lymph nodes proliferated in culture from 2.8-folds to 120-folds.
2. When TIL as well as A-PBL were cultured in 1,000 U/ml of rIL-2, TIL significantly proliferated than A-PBL(P<0.05).
3. Cervical TIL, tumor involved LNL. Normal LNL and A-PBL did not demonstrate any significant cytotoxicity against autologous, allogeneic and K 562 target tumor cells, but autologous lymphokine activated killer(LAK) cells demonstrated antitumor
cytotoxicity against autologous tumor cells although these lysis were low. But, all of 3 rIL-2 cultured cervical TIL demonstrated substantial levels of cytotoxicity against autologous tumor cells(21.8±3.0%) compared to the cytotoxicity of TIL
cultured
without rIL-2(9.1±0.1%) (P<0.05).
4. TIL and tumor involved LNL were predominantly cytotoxic/suppressor T-lymphocytes with an avrage of 53.2% CD 8+ in TIL, 54.7% CD 8+ in tumor involved LNL. The proportion of CD 8+ of TIL and tumor involved LNL were significantly higher than
that
of
normal draining LNL and PBL (P<0.01). There were less thatn 3% CD 56+ (natural killer cell, NK cell) in both of them.
These results suggest that cervical TIL could be expanded in vitro and reach high levels of antitumor effector function in cultures with rIL-2
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KEYWORD
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